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- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- TA319203 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal NFkB p65 (RelA) Phospho S276 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal NFkB p65 (RelA) Phospho S276 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- RELA
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 80 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-pS529 shows phospho p65 staining in carcinoma cells.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-pS276 shows phospho p65 staining in carcinoma cells. WB of lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS276 p65. Lanes 1-6 lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 lysate from ATCC SCC9 cells. Lane 9 lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TNF Induces phosphorylation of p65 in KBM-5 cells.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Immunochemical's pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- anti-p65 pS276 antibody was diluted 1:500 to detect p65 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
- Validation comment
- IHC
