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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- TA324641 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal NFKBp65 Antibody (C-term S536)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal NFKBp65 Antibody (C-term S536)
- Reactivity
- Porcine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- RELA
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- NFKBp65 Antibody (pS536) (Cat. #TA324641) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NFKBp65 antibody detected the NFKBp65 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of U251 cell stained with NFKBp65 Antibody(C-termS536)(Cat#TA324641).U251 cells were incubated with NFKBp65 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml).NFKBp65 immunoreactivity is localized to Cytoplasm significantly.
- Validation comment
- IF
