Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PAB10288 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10288, RRID:AB_1710307
- Product name
- RELA polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of RELA.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Upregulation of HMG1 leads to melanoma inhibitory activity expression in malignant melanoma cells and contributes to their malignancy phenotype.
Curcumin blocks cytokine-mediated NF-kappa B activation and proinflammatory gene expression by inhibiting inhibitory factor I-kappa B kinase activity.
Dexamethasone attenuates NF-kappa B DNA binding activity without inducing I kappa B levels in rat brain in vivo.
Poser I, Golob M, Buettner R, Bosserhoff AK
Molecular and cellular biology 2003 Apr;23(8):2991-8
Molecular and cellular biology 2003 Apr;23(8):2991-8
Curcumin blocks cytokine-mediated NF-kappa B activation and proinflammatory gene expression by inhibiting inhibitory factor I-kappa B kinase activity.
Jobin C, Bradham CA, Russo MP, Juma B, Narula AS, Brenner DA, Sartor RB
Journal of immunology (Baltimore, Md. : 1950) 1999 Sep 15;163(6):3474-83
Journal of immunology (Baltimore, Md. : 1950) 1999 Sep 15;163(6):3474-83
Dexamethasone attenuates NF-kappa B DNA binding activity without inducing I kappa B levels in rat brain in vivo.
Unlap MT, Jope RS
Brain research. Molecular brain research 1997 Apr;45(1):83-9
Brain research. Molecular brain research 1997 Apr;45(1):83-9
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblot of HeLa cell extract.A predominant band ~65 kDa (arrowhead) corresponding to RELA is observed.All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.The membrane was blocked in 5% dry milk for 2 h.After washing, a 1:5,000 dilution of RELA polyclonal antibody (Cat # PAB10288) was added to the membrane and incubated for 2 h.Washes with buffer were performed 4 times for 5' each.The immunoblot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1 : 2,000 for 1 h.Washes with TBS preceded color development.