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- Antibody Data
- Antigen structure
- References [3]
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- Western blot [1]
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- Product number
- PAB10336 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10336, RRID:AB_1710328
- Product name
- RELA (phospho S276) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of RELA.
- Antigen sequence
QLRRPpSDRELSC- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Series introduction: the transcription factor NF-kappaB and human disease.
Control of oncogenesis and cancer therapy resistance by the transcription factor NF-kappaB.
Biological properties and growth in SCID mice of a new myelogenous leukemia cell line (KBM-5) derived from chronic myelogenous leukemia cells in the blastic phase.
Baldwin AS Jr
The Journal of clinical investigation 2001 Jan;107(1):3-6
The Journal of clinical investigation 2001 Jan;107(1):3-6
Control of oncogenesis and cancer therapy resistance by the transcription factor NF-kappaB.
Baldwin AS
The Journal of clinical investigation 2001 Feb;107(3):241-6
The Journal of clinical investigation 2001 Feb;107(3):241-6
Biological properties and growth in SCID mice of a new myelogenous leukemia cell line (KBM-5) derived from chronic myelogenous leukemia cells in the blastic phase.
Beran M, Pisa P, O'Brien S, Kurzrock R, Siciliano M, Cork A, Andersson BS, Kohli V, Kantarjian H
Cancer research 1993 Aug 1;53(15):3603-10
Cancer research 1993 Aug 1;53(15):3603-10
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- Experimental details
- TNF Induces phosphorylation of RELA in KBM-5 cells.Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using Phospho-RELA S276 polyclonal antibody (Cat # PAB10336).RELA polyclonal antibody (Cat # PAB10288) was used a control to show the presence of total RELA in both the cytoplasmic and nuclear extracts.Phosphorylation of RELA occurs after approximately 10 min of TNF exposure.Migration of phosphorylated RELA into the nucleus occurs within a similar time frame.HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.Personal Communication, Aggarwal BB.
