Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NBP1-77827 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77827, RRID:AB_11023813
- Product name
- Rabbit Polyclonal RelA/NFkB p65 Antibody
- Antibody type
- Polyclonal
- Description
- Delipidation and Defibrination. This phospho specific polyclonal antibody is specific for phosphorylated pS276 human p65. Reactivity with non-phosphorylated p65 is minimal.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Toll-like receptor 2 mediates apolipoprotein CIII-induced monocyte activation.
Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation.
Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation.
Kawakami A, Osaka M, Aikawa M, Uematsu S, Akira S, Libby P, Shimokado K, Sacks FM, Yoshida M
Circulation research 2008 Dec 5;103(12):1402-9
Circulation research 2008 Dec 5;103(12):1402-9
Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation.
Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6490-7
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6490-7
Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation.
Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6490-7
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6490-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NFkB p65 [p Ser276] Antibody [NBP1-77827] - Probed against Normal (Lane 1) and TNFalpha (Lane 2) Stimulated HeLa whole cell lysates. A band was observed between 55 and 72 kD corresponding to the expected MW of NFkB p65. The observed higher MW bands have not been characterized.