Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- MA5-15563 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFkB p65 Monoclonal Antibody (6H7)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15563 targets NF-κ-B p65 in indirect ELISA, WB applications and shows reactivity with Human and mouse samples. The MA5-15563 immunogen is purified recombinant fragment of human NF-κ-B p65 expressed in E. Coli. MA5-15563 detects NF-κ-B p65 which has a predicted molecular weight of approximately 65kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6H7
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The Preeclamptic Environment Promotes the Activation of Transcription Factor Kappa B by P53/RSK1 Complex in a HTR8/SVneo Trophoblastic Cell Line.
The anesthetic sevoflurane induces tau trafficking from neurons to microglia.
Salmonella Engages Host MicroRNAs To Modulate SUMOylation: a New Arsenal for Intracellular Survival.
Sakowicz A, Bralewska M, Pietrucha T, Figueras F, Habrowska-Górczyńska DE, Piastowska-Ciesielska AW, Gach A, Sakowicz B, Rybak-Krzyszkowska M, Huras H, Grzesiak M, Biesiada L
International journal of molecular sciences 2021 Sep 22;22(19)
International journal of molecular sciences 2021 Sep 22;22(19)
The anesthetic sevoflurane induces tau trafficking from neurons to microglia.
Dong Y, Liang F, Huang L, Fang F, Yang G, Tanzi RE, Zhang Y, Quan Q, Xie Z
Communications biology 2021 May 12;4(1):560
Communications biology 2021 May 12;4(1):560
Salmonella Engages Host MicroRNAs To Modulate SUMOylation: a New Arsenal for Intracellular Survival.
Verma S, Mohapatra G, Ahmad SM, Rana S, Jain S, Khalsa JK, Srikanth CV
Molecular and cellular biology 2015 Sep 1;35(17):2932-46
Molecular and cellular biology 2015 Sep 1;35(17):2932-46
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NF-Kappa-B p65 using NF-Kappa-B p65 monoclonal antibody (Product # MA5-15563) in Jurkat (1), K562 (2) and NIH/3T3 (3) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NF-Kappa-B p65 using NF-Kappa-B p65 monoclonal antibody (Product # MA5-15563) in Jurkat (1), K562 (2) and NIH/3T3 (3) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NF-Kappa-B p65 using NF-Kappa-B p65 monoclonal antibody (Product # MA5-15563) in Jurkat (1), K562 (2) and NIH/3T3 (3) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Western blot results. ( a ) inhibition of p53 by its targeted siRNA; Western blot results for the cytoplasmic fraction, ( b ) the differences in relative level of pNFkB Ser536 to Histone H3 in nucleus of HTR8/SVneo cells. Cells were cultured for 96 h in hypoxic or normoxic conditions with medium containing 1% serum from preeclamptic or normotensive women. For the first 48 h, the cells were incubated only with medium containing the maternal serum. After 48 h and 72 h, the cell medium was supplemented with the following factors: none i.e., cells without siRNA (1), non-targeting siRNA (2) and targeted siRNA against mRNA coding for p53 (3). The relative level of pNFkB Ser536 to Histone H3 in the nucleus was analyzed. Values are presented as mean +- SEM; ( n = 3experiments). The difference in pNFkB Ser536 level between cells stimulated by negative and targeted siRNA was calculated by the Student's t -test; ## p < 0.01. The difference in pNFkB Ser536 level between the four culture types (i.e., oxygen tension and serum type) which were not stimulated with siRNA were analyzed by ANOVA with the Bonferroni post hoc test; ** p < 0.01, *** p < 0.001. ( c ) Western blot results for nuclear fraction containing pNFkB Ser536 and Histone H3, as a loading control.