R1009

antibody from Acris Antibodies GmbH

Targeting: RELA NFKB3, p65

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunoelectron microscopy (Recommended by provider)

Gel shift (Recommended by provider)

Antibody Data

Product number

R1009

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1009, RRID:AB_1005675

Product name

anti RELA / NF-kB p65

Antibody type

Polyclonal

Antigen

Human NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

0.1 ml

Concentration

80.0 mg/ml (by Refractometry)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Immunoblot of HeLa cell extract. A predominant band ~65 kDa (arrowhead) corresponding to NFkb p65 Rel A is observed. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The immunoblot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.