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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- R1171 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1171, RRID:AB_1005684
- Product name
- anti RELA / NF-kB p65 pSer529
- Antibody type
- Polyclonal
- Antigen
- NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 529 of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH). Sequence information: PNGLLpSGDEDFC
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 ml
- Concentration
- 75.0 mg/ml (by Refractometry)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-Human pSer276 p65.  pan reactive anti p65 (Cat.-No R1009) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Goat-anti-Rabbit IgG was used to develop the western blot using a chemi-luminescent detection method. Other detection methods will yield similar results. Personal Communication, Aggarwal BB
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Anti-pS529 shows phospho p65 staining in carcinoma cells
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Anti-Human pS276 p65 Antibody - Western Blot. TNF Induces phosphorylation of p65 in KBM-5 cells.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Anti-pS276 shows phospho p65 staining in carcinoma cells
