R1487P

antibody from Acris Antibodies GmbH

Targeting: RELA NFKB3, p65

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Immunoelectron microscopy (Recommended by provider)

Gel shift (Recommended by provider)

Antibody Data

Product number

R1487P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1487P, RRID:AB_1005681

Product name

anti RELA / NF-kB p65 (NLS specific)

Antibody type

Polyclonal

Antigen

This antibody was purified from whole rabbit serum prepared by repeated immunizations with the NFκB p65 peptide corresponding to the NLS of the human protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Reactivity

Human

Host

Rabbit

Vial size

0.1 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2. Immunofluorescence microscopy. Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in TNF stimulated DU145 cells. Image shown is at a 1:400 magnification

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Immunohistochemistry.Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in (A) control DU145 cells and (B) TNF stimulated DU145 cells. Although DU145 show relatively high basal levels of nuclear p65 staining, significant enhancement of nuclear staining is seen in pB as evidence of translocation and availability of the NLS tobe bound by the antibody. Cultured cells shown above were formalin-fixed. Tissue staining (not shown) were formalin-fixed, paraffin embedded followed by citrate retrieval. Blocking and hybridization included 5% NGS.