Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP02476PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP02476PU-N, RRID:AB_1619846
- Product name
- anti RELA / NF-kB p65 pSer529
- Antibody type
- Polyclonal
- Antigen
- The antiserum was produced against synthesized non-phosphopeptide derived from human NF-kappaB p65 around the phosphorylation site of serine 529 (L-L-SP-G-D).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 2. Western blot analysis of extracts from MDA-MB-231 cells, untreated or treated with TNF-alpha (20ng/ml, 10min) using NF-kB p65 antibody AP02720PU and NF-kB p65 pSer529 antibody AP02476PU (Line 3 and 4).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 3. Immunofluorescence staining of methanol-fixed HeLa cells using NF-kB p65 pSer529 antibody AP02476PU (Red).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NF-kB p65 pSer529 antibody AP02476PU.