Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-56712 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56712, RRID:AB_838625
- Product name
- Mouse Monoclonal RelA/NFkB p65 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Alveolar Bone Density Reduction in Rats Caused by Unilateral Nasal Obstruction
GR24, a synthetic analog of Strigolactones, alleviates inflammation and promotes Nrf2 cytoprotective response: In vitro and in silico evidences.
Effect of polyhexamethylene biguanide on rat liver.
Liquiritigenin Protects Rats from Carbon Tetrachloride Induced Hepatic Injury through PGC-1α Pathway.
BRCA1-IRIS inactivation overcomes paclitaxel resistance in triple negative breast cancers.
Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model.
Expression of NF-κB, CD68 and CD105 in carotid atherosclerotic plaque.
Green tea polyphenol epigallocatechin-3-gallate inhibits advanced glycation end product-induced expression of tumor necrosis factor-alpha and matrix metalloproteinase-13 in human chondrocytes.
A signal network involving coactivated NF-kappaB and STAT3 and altered p53 modulates BAX/BCL-XL expression and promotes cell survival of head and neck squamous cell carcinomas.
NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins.
Cell-cycle regulators, bcl-2 and NF-kappaB in Epstein-Barr virus-positive gastric carcinomas.
Human keratinocytes respond to interleukin-18: implication for the course of chronic inflammatory skin diseases.
Wang X, Cao Y, Liu Z, Wang Z, Chu X, Wang L, Hu X, Zhao H, Diao Z, Peng F, Ye H, Cao Z
Balkan medical journal 2019 Oct 28;36(6):311-319
Balkan medical journal 2019 Oct 28;36(6):311-319
GR24, a synthetic analog of Strigolactones, alleviates inflammation and promotes Nrf2 cytoprotective response: In vitro and in silico evidences.
Tumer TB, Yılmaz B, Ozleyen A, Kurt B, Tok TT, Taskin KM, Kulabas SS
Computational biology and chemistry 2018 Oct;76:179-190
Computational biology and chemistry 2018 Oct;76:179-190
Effect of polyhexamethylene biguanide on rat liver.
Chowdhury A, Arnold LL, Wang Z, Pennington KL, Dodmane P, Farragut-Cardoso AP, Klaunig JE, Cros D, Creppy EE, Cohen SM
Toxicology letters 2018 Mar 15;285:94-103
Toxicology letters 2018 Mar 15;285:94-103
Liquiritigenin Protects Rats from Carbon Tetrachloride Induced Hepatic Injury through PGC-1α Pathway.
Zhang Y, He Y, Yu H, Ma F, Wu J, Zhang X
Evidence-based complementary and alternative medicine : eCAM 2015;2015:649568
Evidence-based complementary and alternative medicine : eCAM 2015;2015:649568
BRCA1-IRIS inactivation overcomes paclitaxel resistance in triple negative breast cancers.
Blanchard Z, Paul BT, Craft B, ElShamy WM
Breast cancer research : BCR 2015 Jan 13;17:5
Breast cancer research : BCR 2015 Jan 13;17:5
Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model.
Das U, Manna K, Sinha M, Datta S, Das DK, Chakraborty A, Ghosh M, Saha KD, Dey S
PloS one 2014;9(5):e97599
PloS one 2014;9(5):e97599
Expression of NF-κB, CD68 and CD105 in carotid atherosclerotic plaque.
Ren S, Fan X, Peng L, Pan L, Yu C, Tong J, Zhang W, Liu P
Journal of thoracic disease 2013 Dec;5(6):771-6
Journal of thoracic disease 2013 Dec;5(6):771-6
Green tea polyphenol epigallocatechin-3-gallate inhibits advanced glycation end product-induced expression of tumor necrosis factor-alpha and matrix metalloproteinase-13 in human chondrocytes.
Rasheed Z, Anbazhagan AN, Akhtar N, Ramamurthy S, Voss FR, Haqqi TM
Arthritis research & therapy 2009;11(3):R71
Arthritis research & therapy 2009;11(3):R71
A signal network involving coactivated NF-kappaB and STAT3 and altered p53 modulates BAX/BCL-XL expression and promotes cell survival of head and neck squamous cell carcinomas.
Lee TL, Yeh J, Friedman J, Yan B, Yang X, Yeh NT, Van Waes C, Chen Z
International journal of cancer 2008 May 1;122(9):1987-98
International journal of cancer 2008 May 1;122(9):1987-98
NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins.
Santhi WS, Sebastian P, Varghese BT, Prakash O, Pillai MR
Experimental and molecular pathology 2006 Oct;81(2):123-30
Experimental and molecular pathology 2006 Oct;81(2):123-30
Cell-cycle regulators, bcl-2 and NF-kappaB in Epstein-Barr virus-positive gastric carcinomas.
Chang MS, Lee HS, Jung EJ, Kim CW, Lee BL, Kim WH
International journal of oncology 2005 Nov;27(5):1265-72
International journal of oncology 2005 Nov;27(5):1265-72
Human keratinocytes respond to interleukin-18: implication for the course of chronic inflammatory skin diseases.
Wittmann M, Purwar R, Hartmann C, Gutzmer R, Werfel T
The Journal of investigative dermatology 2005 Jun;124(6):1225-33
The Journal of investigative dermatology 2005 Jun;124(6):1225-33
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Lane view shows a specific band for NFkB p65 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 ug/mL mouse anti-RelA/NFkB p65 monoclonal (NB100-56712, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG (HAF018).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis of P65 using p65 antibody at 2 ug/mL in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Detection of p65 by Simple Western (JESS) in chemiluminescence in nuclear extract of reconstructed human epidermis lysate (about 600 ug/mL protein concentration). Antibody at 1:20. Simple Western image submitted by a verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Ovarian cystadenocarcinoma probed with p65 antibody at 5 ug/mL. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis using Azide Free version of NB100-56712. Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents the isotype control (p65) antibody. this antibody was used for this test, and an anti-mouse IgG PE conjugate