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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ARG54079 - Provider product page
- Provider
- Arigo
- Product name
- anti-Fyn antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human Fyn protein fragments expressed in E.coli.
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4B8-E7-A8
- Vial size
- 100 µl
- Concentration
- 10 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Fyn in HeLa, 3T3, C6, COS7, CEM, Ramos and Jurkat cell lysates stained with Fyn antibody [4B8-E7-A8] (ARG54079) (diluted in 1: 500). Predicted band size: 59KDa. Observed band size: 59KDa.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells fixed with -20¢J Methanol and stained with Fyn antibody [4B8-E7-A8] (ARG54079) (diluted in 1:50).
