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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
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- Product number
- SM3108P - Provider product page
- Provider
- OriGene
- Product name
- FYN mouse monoclonal antibody, clone FYN-01, Purified
- Antibody type
- Monoclonal
- Description
- FYN mouse monoclonal antibody, clone FYN-01, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FYN
- Isotype
- IgG
- Antibody clone number
- FYN-01
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 4. Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with anti-Fyn (FYN-01). Lane 1: original lysate of T cells. Lane 2-3: Immunoprecipitated material eluted from affinity sorbent (FYN-01 coupled to Sepharose beads). Lanes differ in amount of T cell lysate loaded on the immunosorbent.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 3. Immunofluorescence staining of Fyn in human primary fibroblasts using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 2. Immunofluorescence staining of Fyn in human HeLa cell line using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 1. Immunofluorescence staining of Fyn in murine transformed fibroblasts using anti-Fyn (FYN-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).
- Validation comment
- IF
