PAB12800

antibody from Abnova Corporation

Targeting: NCK1 NCK, NCKalpha

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB12800

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB12800, RRID:AB_10553392

Product name

NCK1/NCK2 polyclonal antibody

Antibody type

Polyclonal

Antigen

Recombinant His fusion protein corresponding to human NCK1/NCK2.

Reactivity

Human

Host

Rabbit

Vial size

100 uL

Concentration

1 mg/mL

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Nck in a complex containing the catalytic subunit of protein phosphatase 1 regulates eukaryotic initiation factor 2alpha signaling and cell survival to endoplasmic reticulum stress.

Latreille M, Larose L
The Journal of biological chemistry 2006 Sep 8;281(36):26633-44

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The phosphotyrosine peptide binding specificity of Nck1 and Nck2 Src homology 2 domains.

Frese S, Schubert WD, Findeis AC, Marquardt T, Roske YS, Stradal TE, Heinz DW
The Journal of biological chemistry 2006 Jun 30;281(26):18236-45

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The murine Nck SH2/SH3 adaptors are important for the development of mesoderm-derived embryonic structures and for regulating the cellular actin network.

Bladt F, Aippersbach E, Gelkop S, Strasser GA, Nash P, Tafuri A, Gertler FB, Pawson T
Molecular and cellular biology 2003 Jul;23(13):4586-97

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoblot analysis of cell lysates.Lane 1 : U937 cell lysate. Lnae 2 : A-431 cell lysate.Lane 3 : NIH/3H3 cell lysate.Lane 4 : C6 cell lysate.