TA324086

antibody from OriGene

Targeting: NCK1 NCK, NCKalpha

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324086

Provider

OriGene

Product name

Anti-NCK1 Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Anti-NCK1 Rabbit Polyclonal Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

NCK1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1.2mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 43 kDa . Positive control: Jurkat and K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: K562 cell lysate Lysates: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using NCK1 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x200)

Validation comment

IHC