AM06766PU-N

antibody from OriGene

Targeting: NCK1 NCK, NCKalpha

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM06766PU-N

Provider

OriGene

Product name

Nck (NCK1) mouse monoclonal antibody, clone 5B7, Purified

Antibody type

Monoclonal

Description

Nck (NCK1) mouse monoclonal antibody, clone 5B7, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

NCK1

Isotype

IgG

Antibody clone number

5B7

Vial size

100 µg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis using NCK1 mAb against human NCK1 (AA: 203-371) recombinant protein (Expected MW is 44.9 kDa)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NCK1 mouse mAb with DAB staining.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells using NCK1 mouse mAb (green) and negative control (red).

Validation comment

FC