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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-29232 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: K562.
- Concentration
- 0.78 mg/mL
Submitted references Expression and clinicopathological significance of Nck1 in human astrocytoma progression.
Deshpande RP, Panigrahi M, Y B V K CS, Babu PP
The International journal of neuroscience 2019 Feb;129(2):171-178
The International journal of neuroscience 2019 Feb;129(2):171-178
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NCK1 Polyclonal Antibody (Product # PA5-29232). Sample (30 µg of whole cell lysate). Lane A: K562 . 10% SDS PAGE. NCK1 Polyclonal Antibody (Product # PA5-29232) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NCK1 in paraformaldehyde-fixed A431 cells using a NCK1 polyclonal antibody (Product # PA5-29232) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Hela xenograft, using NCK1 (Product # PA5-29232) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. Western blotting (A) Nck1 (1:1500 dilution) and densitometric analysis (B) of Nck1 in control, low-grade (GII) and high-grade (GIII, GIV) tumor tissue samples. Protein expression in GIV tissues was significantly ( p < 0.05) increased compared with control (a), GII (b), and GIII (c). Internal Nck1 expression were normalized with Beta actin (internal control) C = control brain tissue, GII = Grade-II astrocytoma tissue, GIII = Grade-III astrocytoma tissue, and GIV = Grade-IV astrocytoma tissue.
