- GTX109679 - GeneTex HDAC3 antibody

Submitted references NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Huang D, Chen J, Yang L, Ouyang Q, Li J, Lao L, Zhao J, Liu J, Lu Y, Xing Y, Chen F, Su F, Yao H, Liu Q, Su S, Song E
Nature immunology 2018 Oct;19(10):1112-1125

Inhibition of HDAC3- and HDAC6-Promoted Survivin Expression Plays an Important Role in SAHA-Induced Autophagy and Viability Reduction in Breast Cancer Cells.

Lee JY, Kuo CW, Tsai SL, Cheng SM, Chen SH, Chan HH, Lin CH, Lin KY, Li CF, Kanwar JR, Leung EY, Cheung CC, Huang WJ, Wang YC, Cheung CH
Frontiers in pharmacology 2016;7:81

Exercise increases the binding of MEF2A to the Cpt1b promoter in mouse skeletal muscle.

Yuan H, Niu Y, Liu X, Fu L
Acta physiologica (Oxford, England) 2014 Dec;212(4):283-92

NudC deacetylation regulates mitotic progression.

Chuang C, Pan J, Hawke DH, Lin SH, Yu-Lee LY
PloS one 2013;8(9):e73841

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: <50%
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
KD Reagent Prod page: KD Reagent Prod page
Antibody Lot Number: 39974

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample(30 ?g whole cell lysate)A: 293TB: A431 (GTX27909)C: H1299D: HeLa S3 (GTX14654)E: HepG2 (GTX27900)F: MOLT4 (GTX27912)G: Raji (GTX27908)10% SDS PAGEGTX109679 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term (GTX109679) diluted at 1:400.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HDAC3 antibody immunoprecipitates HDAC3 protein in IP experiments. IP Sample: 1000 ?g 293T whole cell lysate/extract A. 50 £gg 293T whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 £gg of HDAC3 antibody (GTX109679) 10% SDS-PAGE The immunoprecipitated HDAC3 protein was detected by HDAC3 antibody (GTX109679) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HDAC3(GTX109679) antibody at 1:500 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HDAC3 antibody immunoprecipitates HDAC3 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 £gg preimmune rabbit IgG B. 5 £gg of HDAC3 antibody (GTX109679) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

GTX109679

Antibody data