PAB11252

antibody from Abnova Corporation

Targeting: BBC3 JFY1, PUMA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB11252

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB11252, RRID:AB_1671641

Product name

BBC3 polyclonal antibody

Antibody type

Polyclonal

Antigen

A synthetic peptide corresponding to C-terminus of human BBC3.

Reactivity

Human, Mouse

Host

Rabbit

Vial size

100 μg

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals.

Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T
Proceedings of the National Academy of Sciences of the United States of America 2001 Sep 25;98(20):11318-23

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PUMA, a novel proapoptotic gene, is induced by p53.

Nakano K, Vousden KH
Molecular cell 2001 Mar;7(3):683-94

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PUMA induces the rapid apoptosis of colorectal cancer cells.

Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B
Molecular cell 2001 Mar;7(3):673-82

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of BBC3 expression in human K-562 (lane 1) and mouse NIH/3T3 (lane 2) cell lysates with BBC3 polyclonal antibody (Cat # PAB11252) at 2 ug/mL .

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunocytochemistry of BBC3 in K-562 cells with BBC3 polyclonal antibody (Cat # PAB11252) at 1 ug/mL .

Validation comment

Immunocytochemistry