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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [5]
- Flow cytometry [1]
- Other assay [3]
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- Product number
- 44-1092G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-GYS1 (Ser641, Ser645) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Fibroblast growth factor 21 improves hepatic insulin sensitivity by inhibiting mammalian target of rapamycin complex 1 in mice.
Accelerated recovery of mitochondrial membrane potential by GSK-3β inactivation affords cardiomyocytes protection from oxidant-induced necrosis.
Translocation of glycogen synthase kinase-3β (GSK-3β), a trigger of permeability transition, is kinase activity-dependent and mediated by interaction with voltage-dependent anion channel 2 (VDAC2).
Glycogen synthase kinase-3 inactivation is not required for ischemic preconditioning or postconditioning in the mouse.
Gong Q, Hu Z, Zhang F, Cui A, Chen X, Jiang H, Gao J, Chen X, Han Y, Liang Q, Ye D, Shi L, Chin YE, Wang Y, Xiao H, Guo F, Liu Y, Zang M, Xu A, Li Y
Hepatology (Baltimore, Md.) 2016 Aug;64(2):425-38
Hepatology (Baltimore, Md.) 2016 Aug;64(2):425-38
Accelerated recovery of mitochondrial membrane potential by GSK-3β inactivation affords cardiomyocytes protection from oxidant-induced necrosis.
Sunaga D, Tanno M, Kuno A, Ishikawa S, Ogasawara M, Yano T, Miki T, Miura T
PloS one 2014;9(11):e112529
PloS one 2014;9(11):e112529
Translocation of glycogen synthase kinase-3β (GSK-3β), a trigger of permeability transition, is kinase activity-dependent and mediated by interaction with voltage-dependent anion channel 2 (VDAC2).
Tanno M, Kuno A, Ishikawa S, Miki T, Kouzu H, Yano T, Murase H, Tobisawa T, Ogasawara M, Horio Y, Miura T
The Journal of biological chemistry 2014 Oct 17;289(42):29285-96
The Journal of biological chemistry 2014 Oct 17;289(42):29285-96
Glycogen synthase kinase-3 inactivation is not required for ischemic preconditioning or postconditioning in the mouse.
Nishino Y, Webb IG, Davidson SM, Ahmed AI, Clark JE, Jacquet S, Shah AM, Miura T, Yellon DM, Avkiran M, Marber MS
Circulation research 2008 Aug 1;103(3):307-14
Circulation research 2008 Aug 1;103(3):307-14
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Peptide Competition and Phosphatase Treatment Lysates prepared from 3T3-L1 cells left unstimulated (1), stimulated with Wortmanin for 90 minutes (2), stimulated with Wortmanin for 90 minutes plus insulin for 60 minutes (3), stimulated with insulin for 20
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Peptide Competition and Phosphatase Treatment Lysates prepared from 3T3-L1 cells left unstimulated (1), stimulated with Wortmanin for 90 minutes (2), stimulated with Wortmanin for 90 minutes plus insulin for 60 minutes (3), stimulated with insulin for 20
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), U-87 MG treated with Cobalt chloride (150uM for 24hrs) (Lane 2), L6 (Lane 3), L6 treated with Insulin (10nM for 24hrs) (Lane 4), MCF-7 (Lane 5) and MCF-7 treated with Cobalt chloride (Lane 6). The blot was probed with Anti-Phospho-GYS1 (Ser641, Ser645) Antibody (Product # 44-1092G, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 84 kDa band corresponding to Phospho-GYS1 (Ser641, Ser645) was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-GYS1 at Ser641, Ser645 was performed by loading whole-cell lysates of four different human cell lines. Phospho-GYS1 was detected at approximately 85kDa by using a Phospho-GYS1 polyclonal Antibody (Product # 44-1092G) at a dilution of 1:500 in Blocking Buffer overnight at 4°C on a rocking platform, followed by a 1-hour room-temperature incubation of a donkey anti-rabbit IgG antibody at a dilution of 1:5000. Data courtesy of Thermo Scientific KOL Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-GYS1 at Ser641, Ser645 was performed by loading whole-cell lysates of mouse tissue samples. Phospho-GYS1 was detected at approximately 85kDa by using a Phospho-GYS1 polyclonal Antibody (Product # 44-1092G) at a dilution of 1:500 in Blocking Buffer overnight at 4°C on a rocking platform, followed by a 1-hour room-temperature incubation of a donkey anti-rabbit IgG antibody at a dilution of 1:5000. Data courtesy of Thermo Scientific KOL Program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Phospho-Glycogen Synthase [pSer641/pSer645] was done on HeLa cells treated with PMA (200nM, 20 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Phospho-Glycogen Synthase [pSer641/pSer645] Rabbit Polyclonal Antibody (441092G, red histogram) or with rabbit isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Effects of inhibition of GSK-3beta on antimycin A-induced changes in DeltaPsi m and its recovery. A: Western blotting for Ser9-phospho- and total GSK-3beta in mitochondria, B: Effects of antimycin A (AA) on phospho-GSK-3beta level. C: TMRE fluorescence after AA treatment in vehicle- and LiCl-pretreated cells. Western blotting for Ser9-phospho-GSK-3beta, total GSK-3beta, Ser641/645-phospho-glycogen synthase (GS), non-phospho-GS and beta-actin (loading control) in total lysates of vehicle-treated and LiCl-treated cells. Treatments with 30 mM and 60 mM LiCl for 60 min induced phosphorylation of GSK-3beta and dephosphorylation of GS. Increased phosphorylation of GSK-3beta by LiCl reflects reduced activity of protein phosphatase 1, which is positively regulated by GSK-3beta activity. D: TMRE fluorescence after AA treatment in control siRNA- and GSK-3beta-siRNA-pretreated cells. NC = nicorandil. Treatment = time after onset of treatment with AA, Washout = time after washout of AA. *p
