PAB10328

antibody from Abnova Corporation

Targeting: GYS1 GSY, GYS

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10328

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10328, RRID:AB_1708166

Product name

GYS1 (phospho S640) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of GYS1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoblotting of GYS1 (phospho S640) polyclonal antibody (Cat # PAB10328) to human GYS1 at pS640 was used at a 1 : 1000 dilution to detect human muscle GYS1 by Western blot.Approximately 12 uL of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE.Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 uM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4 nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'.After washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.