10566-1-AP

antibody from Invitrogen Antibodies

Targeting: GYS1 GSY, GYS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10566-1-AP

Provider

Invitrogen Antibodies

Product name

GYS1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: RAIFATQRQ SFPPVCTHNM LDDSSDPILT TIRRIGLFNS SADRVKVIFH PEFLSSTSPL LPVDYEEFVR GCHLGVFPSY YEPWGYTPAE CTVMGIPSIS TNLSGFGCFM EEHIADPSAY GIYILDRRFR SLDDSCSQLT SFLYSFCQQS RRQRIIQRNR TERLSDLLDW KYLGRYYMSA RHMALSKAFP EHFTYEPNEA DAAQGYRYPR PASVPPSPSL SRHSSPHQSE DEEDPRNGPL EEDGERYDED EEAAKDRRNI RAPEWPRRAS CTSSTSGSKR NSVDTATSSS LSTPSEPLSP TSSLGEERN (430-737 aa encoded by BC007688)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.36 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 10566-1-AP (GYS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 10566-1-AP (GYS1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10566-1-AP (GYS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 10566-1-AP (GYS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using GYS1 antibody 10566-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 10566-1-AP (GYS1 antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 10566-1-AP (GYS1 antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 10566-1-AP (GYS1 antibody) at dilution of 1:50 (under 25x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 10566-1-AP ( GYS1 antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 10566-1-AP ( GYS1 antibody) at dilution of 1:200 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug GYS1 antibody (10566-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-GYS1 (IP:10566-1-AP, 4ug; Detection:10566-1-AP 1:300) with mouse skeletal muscle tissue lysate 2200ug.