NBP2-00532

antibody from Novus Biologicals

Targeting: BCAR1 CAS, CASS1, Crkas, P130Cas

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-00532

Provider

Novus Biologicals

Product name

Mouse Monoclonal p130Cas/BCAR1 Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human, Mouse, Rat, Canine, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

0.53 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BCAR1 Antibody (1A9) [NBP2-00532] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAR1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BCAR1 Antibody (1A9) [NBP2-00532] Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BCAR1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BCAR1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BCAR1 mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: BCAR1 Antibody (1A9) [NBP2-00532] - Analysis of Hela cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: BCAR1 Antibody (1A9) [NBP2-00532] - Analysis of Jurkat cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: BCAR1 Antibody (1A9) [NBP2-00532] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BCAR1 antibody, and then analyzed by flow cytometry.