F41629

antibody from NSJ Bioreagents

Targeting: CDKN2A ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F41629

Provider

NSJ Bioreagents

Product name

p16INK4a Antibody

Antibody type

Polyclonal

Antigen

A portion of amino acids 104-131 from the human protein was used as the immunogen for this p16INK4a antibody.

Description

Antigen affinity purified antibody

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

80 µl, 400 µl

Storage

Aliquot the p16INK4a antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of lysate from A2058 cell line using p16INK4a antibody at 1:1000. Predicted molecular weight: 16 kDa

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of p16INK4a antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

p16INK4a antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

p16INK4a antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.