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LearnCF506867
antibody from Invitrogen Antibodies
Targeting: UPRT
DKFZp781E1243, FUR1, MGC23937, RP11-311P8.3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- CF506867 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UPRT Monoclonal Antibody (OTI2D10), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2D10
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UPRT (RC205030, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-UPRT. Positive lysates LY408068 (100 µg) and LC408068 (20 µg) can be purchased separately from OriGene.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-UPRT mouse monoclonal antibody (TA506867) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UPRT(RC205030).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-UPRT mouse monoclonal antibody (TA506867) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UPRT(RC205030).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-UPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506867)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-UPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506867)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human prostate tissue within the normal limits using anti-UPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506867)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506867)
