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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
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- Product number
- AM3111a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM3111a, RRID:AB_2107702
- Product name
- Gab1 Antibody (Ascites)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.35 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Role of Gab1 in UV-induced c-Jun NH2-terminal kinase activation and cell apoptosis.
Sun Y, Yuan J, Liu H, Shi Z, Baker K, Vuori K, Wu J, Feng GS
Molecular and cellular biology 2004 Feb;24(4):1531-9
Molecular and cellular biology 2004 Feb;24(4):1531-9
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Wild-type (+/+) and Gab1-/- (-/-) cells were heat shocked (HS) at 42°C for 1 h or irradiated with UV-B light (400 J/m2) and then incubated at 37? for 1 h. Cell lysates were prepared and subjected to immunoprecipitation (IP) with an anti-JNK2 antibody. The immunoprecipitates were resolved by SDS- PAGE and immunoblotted (IB) with anti-Gab1 antibody. Data courtesy of Dr. GS Feng at The Burnham Institute.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Rescue of the JNK pathway by expression of wild-type Gab1 in Gab1-/- cells. Gab1-/- cells were transiently transfected with 1, 2, or 4 of the expression construct for human Gab1 cDNA, using the GenePORTER 2 transfection reagent (Gene Therapy Systems Inc.). After incubation, the cells were irradiated with UV-B light at 400 J/m2 or left untreated. Exogenous expression of Gab1 in transfected cells was confirmed by anti-Gab1 immunoblot analysis with wild-type cells as positive control. +/+, wild-type cells; -/-, Gab1-/- cells. (Mol. Cell. Biol. 2004 Feb 15;24(4):1531-1539)"
- Primary Ab dilution
- 1:1000
