GTX26276

antibody from GeneTex

Targeting: ACTB

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX26276

Provider

GeneTex

Proper citation

GeneTex Cat#GTX26276, RRID:AB_367161

Product name

beta Actin antibody [AC-15]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Feline, Guinea Pig, Porcine, Rabbit, Sheep, Simian

Host

Mouse

References

Submitted references

Chloroquine inhibits human retina pigmented epithelial cell growth and microtubule nucleation by downregulating p150^glued.

Chen TY, Lien WC, Cheng HL, Kuan TS, Sheu SY, Wang CY
Journal of cellular physiology 2019 Jul;234(7):10445-10457

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Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses.

Bhattacharjee P, Keyel PA
Scientific reports 2018 Apr 24;8(1):6458

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BDNF/trkB Induction of Calcium Transients through Ca(v)2.2 Calcium Channels in Motoneurons Corresponds to F-actin Assembly and Growth Cone Formation on β2-Chain Laminin (221).

Dombert B, Balk S, Lüningschrör P, Moradi M, Sivadasan R, Saal-Bauernschubert L, Jablonka S
Frontiers in molecular neuroscience 2017;10:346

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Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer.

Shan YS, Hsu HP, Lai MD, Hung YH, Wang CY, Yen MC, Chen YL
Oncology letters 2017 Oct;14(4):4517-4526

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Differential roles of α-, β-, and γ-actin in axon growth and collateral branch formation in motoneurons.

Moradi M, Sivadasan R, Saal L, Lüningschrör P, Dombert B, Rathod RJ, Dieterich DC, Blum R, Sendtner M
The Journal of cell biology 2017 Mar 6;216(3):793-814

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Hypermethylation of BEND5 contributes to cell proliferation and is a prognostic marker of colorectal cancer.

Lin RK, Hung WY, Huang YF, Chang YJ, Lin CH, Chen WY, Chiu SF, Chang SC, Tsai SF
Oncotarget 2017 Dec 26;8(69):113431-113443

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Characterization of a novel p110β-specific inhibitor BL140 that overcomes MDV3100-resistance in castration-resistant prostate cancer cells.

He C, Duan S, Dong L, Wang Y, Hu Q, Liu C, Forrest ML, Holzbeierlein JM, Han S, Li B
The Prostate 2017 Aug;77(11):1187-1198

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Disruption of a Structurally Important Extracellular Element in the Glycine Receptor Leads to Decreased Synaptic Integration and Signaling Resulting in Severe Startle Disease.

Schaefer N, Berger A, van Brederode J, Zheng F, Zhang Y, Leacock S, Littau L, Jablonka S, Malhotra S, Topf M, Winter F, Davydova D, Lynch JW, Paige CJ, Alzheimer C, Harvey RJ, Villmann C
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Aug 16;37(33):7948-7961

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IRS4 induces mammary tumorigenesis and confers resistance to HER2-targeted therapy through constitutive PI3K/AKT-pathway hyperactivation.

Ikink GJ, Boer M, Bakker ER, Hilkens J
Nature communications 2016 Nov 23;7:13567

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Differential expression of TGF-β superfamily members and role of Smad1/5/9-signalling in chondral versus endochondral chondrocyte differentiation.

Dexheimer V, Gabler J, Bomans K, Sims T, Omlor G, Richter W
Scientific reports 2016 Nov 16;6:36655

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Modulation of extracellular signal-related kinase, cyclin D1, glial fibrillary acidic protein, and vimentin expression in estradiol-pretreated astrocyte cultures treated with competence and progression growth factors.

Bramanti V, Grasso S, Tibullo D, Giallongo C, Raciti G, Viola M, Avola R
Journal of neuroscience research 2015 Sep;93(9):1378-87

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Bax/Bak-dependent, Drp1-independent Targeting of X-linked Inhibitor of Apoptosis Protein (XIAP) into Inner Mitochondrial Compartments Counteracts Smac/DIABLO-dependent Effector Caspase Activation.

Hamacher-Brady A, Brady NR
The Journal of biological chemistry 2015 Sep 4;290(36):22005-18

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Regulation of PACT-Mediated Protein Kinase Activation by the OV20.0 Protein of Orf Virus.

Tseng YY, Liao GR, Sen GC, Lin FY, Hsu WL
Journal of virology 2015 Nov;89(22):11619-29

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Functional analysis of the short isoform of orf virus protein OV20.0.

Tseng YY, Lin FY, Cheng SF, Tscharke D, Chulakasian S, Chou CC, Liu YF, Chang WS, Wong ML, Hsu WL
Journal of virology 2015 May;89(9):4966-79

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Elastase induces lung epithelial cell autophagy through placental growth factor: a new insight of emphysema pathogenesis.

Hou HH, Cheng SL, Chung KP, Kuo MY, Yeh CC, Chang BE, Lu HH, Wang HC, Yu CJ
Autophagy 2014 Sep;10(9):1509-21

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Intramitochondrial recruitment of endolysosomes mediates Smac degradation and constitutes a novel intrinsic apoptosis antagonizing function of XIAP E3 ligase.

Hamacher-Brady A, Choe SC, Krijnse-Locker J, Brady NR
Cell death and differentiation 2014 Dec;21(12):1862-76

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Expression analysis of Lrrk1, Lrrk2 and Lrrk2 splice variants in mice.

Giesert F, Hofmann A, Bürger A, Zerle J, Kloos K, Hafen U, Ernst L, Zhang J, Vogt-Weisenhorn DM, Wurst W
PloS one 2013;8(5):e63778

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Lysyl 5-hydroxylation, a novel histone modification, by Jumonji domain containing 6 (JMJD6).

Unoki M, Masuda A, Dohmae N, Arita K, Yoshimatsu M, Iwai Y, Fukui Y, Ueda K, Hamamoto R, Shirakawa M, Sasaki H, Nakamura Y
The Journal of biological chemistry 2013 Mar 1;288(9):6053-62

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Modulation of serines 17 and 24 in the LC3-interacting region of Bnip3 determines pro-survival mitophagy versus apoptosis.

Zhu Y, Massen S, Terenzio M, Lang V, Chen-Lindner S, Eils R, Novak I, Dikic I, Hamacher-Brady A, Brady NR
The Journal of biological chemistry 2013 Jan 11;288(2):1099-113

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Analysis of molecular mechanisms and anti-tumoural effects of zoledronic acid in breast cancer cells.

Insalaco L, Di Gaudio F, Terrasi M, Amodeo V, Caruso S, Corsini LR, Fanale D, Margarese N, Santini D, Bazan V, Russo A
Journal of cellular and molecular medicine 2012 Sep;16(9):2186-95

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Arginine deiminase PEG20 inhibits growth of small cell lung cancers lacking expression of argininosuccinate synthetase.

Kelly MP, Jungbluth AA, Wu BW, Bomalaski J, Old LJ, Ritter G
British journal of cancer 2012 Jan 17;106(2):324-32

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Novel mechanistic insights into arginine deiminase pharmacology suggest 18F-FDG is not suitable to evaluate clinical response in melanoma.

Stelter L, Evans MJ, Jungbluth AA, Zanzonico P, Ritter G, Ku T, Rosenfeld E, Bomalaski JS, Old L, Larson SM
Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2012 Feb;53(2):281-6

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Pericytes promote endothelial cell survival through induction of autocrine VEGF-A signaling and Bcl-w expression.

Franco M, Roswall P, Cortez E, Hanahan D, Pietras K
Blood 2011 Sep 8;118(10):2906-17

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Therapeutic effects of PEGylated insulin-like growth factor I in the pmn mouse model of motoneuron disease.

Jablonka S, Holtmann B, Sendtner M, Metzger F
Experimental neurology 2011 Dec;232(2):261-9

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Salbutamol inhibits lipopolysaccharide-induced inflammatory responses in rat peritoneal macrophages.

Tanaka S, Tsutsui M, Kishida T, Souma S, Kuroda J, Yoshida T
The Journal of toxicological sciences 2010 Jun;35(3):327-34

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Identification of major S-nitrosylated proteins in murine experimental autoimmune encephalomyelitis.

Bizzozero OA, Zheng J
Journal of neuroscience research 2009 Oct;87(13):2881-9

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot of cell lysates from whole rat brain (10 μg), untreated rat ventral mesencephalic (VM) cultures, or VM cultures treated with 1 mM db cAMP or 1 mM db cAMP + 1 uM rat galanin (2 μg) for 2 days. Lysates were separated by SDS-PAGE and immunoblott

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Whole cell extract of human fibroblasts was separated on SDS-PAGE and blotted with Monoclonal Anti-£]-Actin (Cat No. GTX26276). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjμgate and AEC substrate. Lanes A: Antibody dilution 1:5,000 B: Negative control (only secondary antibody)

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed HeLa, using beta Actin(GTX26276) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

a-c. Double exposure micrographs of longitudinal, expanded (a,b) and transverse (c) semi-thin cryosections of chicken gizzard muscle double labeled with Monoclonal Anti-£]-Actin, Clone No. AC-15 (Cat. No. GTX26276) (green) and polyclonal antibodies to chicken gizzard myosin (red). d-f. Longitudinal, expanded cryosections of chicken gizzard muscle double labeled with polyclonal Anti-£\-Actinin (green, d, e; red, f) in combination with antibodies to chicken gizzard myosin, antibodies to chicken desmin and Monoclonal Anti-£]-Actin, Clone No. AC-15 (Cat. No. GTX26276) (f, green). Bars, 10 mm.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-£] Actin, Clone No. AC-15 (Cat. No. GTX26276).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ICC/IF analysis of HS-68 cells using beta Actin antibody [AC-15] at a dilution of 1:1,000 (green) and DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IHC-P analysis of human heart tissue using beta Actin antibody [AC-15] at a dilution of 1:1,000.

Other assay

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using beta Actin antibody [AC-15] at a dilution of 1:5,000.

Validation comment

IB