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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX83163 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83163, RRID:AB_11162633
- Product name
- beta Actin antibody [8H10D10]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Hamster, Simian
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis using anti beta-Actin mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12(5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using anti-beta Actin mAb (red, the secondary Ab is Cy3-Goat anti IgG). Blue: DRAQ5 fluorescent DNA dye.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using anti-beta Actin mAb (right) and negative control (left).
