Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AM06276SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM06276SU-N, RRID:AB_11217046
- Product name
- anti Actin beta / ACTB
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide corresponding to amino-terminal residues of Human beta-Actin, conjugated to KLH.
- Reactivity
- Human, Mouse, Rat, Hamster, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8H10D10
- Vial size
- 0.1 ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis using beta-Actin antibody Cat.-No AM06276SU-N against NIH/3T3 (Lane 1), Jurkat (Lane 2), Hela (Lane 3), CHO (Lane 4), PC12 (Lane 5), HEK293 (Lane 6), COS (Lane 7), A549 (Lane 8) and MCF-7 (Lane 9) cell lysate.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal Immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin antibody Cat.-No AM06276SU-N (red, the secondary Ab is Cy3-Goat anti Mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of MCF-7 cells using beta Actin antibody Cat.-No AM06276SU-N (right) and Negative Control (left).