Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-50031 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CNPase Antibody
- Antibody type
- Monoclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CNPase Antibody (1H10) [NBP2-50031] - Western blot analysis of different tissue lysates using mouse mAb to CNP, NBP2-50031, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. A doublet of bands at 46 and 48kDa correspond to the major isotypes of the CNP protein.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry Free-Floating: CNPase Antibody (1H10) [NBP2-50031] - Analysis of rat cerebellum section stained with mouse mAb to CNP, NBP2-50031, dilution 1:500 in green and costained with chicken pAb to NF-M, dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The CNP antibody specifically stains oligodendrocytes, cells that create the myelin sheath around axons. NF-M antibody labels axons of neuronal cells.
