10191-2-AP

antibody from Invitrogen Antibodies

Targeting: DFFA DFF-45, DFF1, DFF45, ICAD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10191-2-AP

Provider

Invitrogen Antibodies

Product name

DFF45/DFFA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: GVPESGEIR TLKPCLLRRN YSREQHGVAA SCLEDLRSKA CDILAIDKSL TPVTLVLAED GTIVDDDDYF LCLPSNTKFV ALASNEKWAY NNSDGGTAWI SQESFDVDET DSGAGLKWKN VARQLKEDLS SIILLSEEDL QMLVDAPCSD LAQELRQSCA TVQRLQHTLQ QVLDQREEVR QSKQLLQLYL QALEKEGSLL SKQEESKAAF GEEVDAVDTG ISRETSSDVA LASHILTALR EKQAPELSLS SQDLELVTKE DPKALAVALN WDIKKTETVQ EACERELALR LQQTQSLHSL RSISASKASP PGDLQNPKRA RQ (8-328 aa encoded by BC000037)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.4 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HEK-293 cells using 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:100 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DFF45/DFFA (IP:10191-2-AP, 3ug; Detection:10191-2-AP 1:1000) with HEK-293 cells lysate 2500ug.