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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF7237 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Syntaxin 1A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat Syntaxin 1A in Western blots and detects recombinant human Syntaxin 1A in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Syntaxin 1B2 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q16623- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Syntaxin 1A by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Syntaxin 1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7237) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Syntaxin 1A at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Syntaxin 1A in SH-SY5Y Human Cell Line. SH-SY5Y human neuroblastoma cells were cultured overnight in the presence of 1 mM Retinoic Acid (Catalog # 0695/50) prior to immersion fixation. Syntaxin 1A was detected using a Goat Anti-Human/Mouse/Rat Syntaxin 1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7237). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Goat IgG Affinity-purified Secondary Antibody (red; Catalog # NL001). Actin filaments were stained with FITC-conjugated Phalloidin (green) and the cell nuclei were counter-stained with DAPI (blue). Syntaxin 1A immunoreactivity was localized to synaptic vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
