H00000860-M01
antibody from Novus Biologicals
Targeting: RUNX2
AML3, CBFA1, CCD, CCD1, PEBP2A1, PEBP2aA1
Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00000860-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000860-M01, RRID:AB_537450
- Product name
- Mouse Monoclonal RUNX2/CBFA1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RUNX2 - runt-related transcription factor 2
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Vitamin D Promotes MSC Osteogenic Differentiation Stimulating Cell Adhesion and αVβ3 Expression.
Breast osteoblast-like cells: a new biomarker for the management of breast cancer.
Processing large-diameter poly(L-lactic acid) microfiber mesh/mesenchymal stromal cell constructs via resin embedding: an efficient histologic method.
Runt-related transcription factor 2 in human colon carcinoma: a potent prognostic factor associated with estrogen receptor.
Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2.
Posa F, Di Benedetto A, Cavalcanti-Adam EA, Colaianni G, Porro C, Trotta T, Brunetti G, Lo Muzio L, Grano M, Mori G
Stem cells international 2018;2018:6958713
Stem cells international 2018;2018:6958713
Breast osteoblast-like cells: a new biomarker for the management of breast cancer.
Scimeca M, Urbano N, Bonfiglio R, Schillaci O, Bonanno E
British journal of cancer 2018 Oct;119(9):1129-1132
British journal of cancer 2018 Oct;119(9):1129-1132
Processing large-diameter poly(L-lactic acid) microfiber mesh/mesenchymal stromal cell constructs via resin embedding: an efficient histologic method.
D'Alessandro D, Pertici G, Moscato S, Metelli MR, Danti S, Nesti C, Berrettini S, Petrini M, Danti S
Biomedical materials (Bristol, England) 2014 Aug;9(4):045007
Biomedical materials (Bristol, England) 2014 Aug;9(4):045007
Runt-related transcription factor 2 in human colon carcinoma: a potent prognostic factor associated with estrogen receptor.
Sase T, Suzuki T, Miura K, Shiiba K, Sato I, Nakamura Y, Takagi K, Onodera Y, Miki Y, Watanabe M, Ishida K, Ohnuma S, Sasaki H, Sato R, Karasawa H, Shibata C, Unno M, Sasaki I, Sasano H
International journal of cancer 2012 Nov 15;131(10):2284-93
International journal of cancer 2012 Nov 15;131(10):2284-93
Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2.
Yang X, Fullerton DA, Su X, Ao L, Cleveland JC Jr, Meng X
Journal of the American College of Cardiology 2009 Feb 10;53(6):491-500
Journal of the American College of Cardiology 2009 Feb 10;53(6):491-500
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Western Blot analysis of RUNX2 expression in SJCRH30 ( Cat # L027V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Western Blot analysis of RUNX2 expression in K-562 ( Cat # L009V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of RUNX2 expression in transfected 293T cell line by RUNX2 monoclonal antibody (M01), clone 1D8.Lane 1: RUNX2 transfected lysate (Predicted MW: 60.28 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - RUNX2 monoclonal antibody (M01), clone 1D8. Analysis of RUNX2 expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Detection limit for recombinant GST tagged RUNX2 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of monoclonal antibody to RUNX2 on U-2 OS cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.