Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- M00149-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for Cyclin D1/CCND1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat Cyclin D1/CCND1 information: Molecular Weight: 33729 MW; Subcellular Localization: Nucleus . Cytoplasm . Membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- DAD-3
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- Immunohistochemical analysis of paraffin-embedded human bladder, using Cyclin D1 Antibody(M00149-1)CCND1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CCND1 Antibody (M00149-1)overnight at 4
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