Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [4]
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Validation data
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- Product number
- ADI-KAM-CC200-E - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ADI-KAM-CC200-E, RRID:AB_2039040
- Product name
- Cyclin D1 monoclonal antibody (5D4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Description
- Protein A affinity purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5D4
- Vial size
- 100 μg
- Storage
- -20°C
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of ZR- 75-1 (1), MCF7 (2), HeLa (3), WR19L (4) and NIH/3T3 (5) cells probed with Cyclin D1 mAb (5D4).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cyclin D1 on paraffin embedded section of human mantle cell lymphoma with Cyclin D1 mAb (5D4).
- Submitted by
- Enzo Life Sciences (provider)
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- Experimental details
- Immunoprecipitation analysis of cyclin D1 from ZR75-1 cells with Mouse IgG2a (1) or Cyclin D1 mAb (5D4) (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoBlotted with 553.
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- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Cyclin D1 expression in HeLa cells. Open histogram indicates the reaction of Isotypic control to the cells. Shaded histogram indicates the reaction of Cyclin D1 mAb (5D4) to the cells.