Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AM26674AF-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti Cyclin D1
- Antibody type
- Monoclonal
- Antigen
- Recombinant human PRAD1/Cyclin D1
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5D4
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Cyclin D1 expression in ZR-75-1 (1), MCF7 (2), HeLa (3), WR19L (4) and NIH/3T3 (5) using AM26674AF-N.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation of Cyclin D1 from ZR-75-1 cells with Mouse IgG2a (1) or AM26674AF-N (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDSPAGE and immunoblotted with 553.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical detection of Cyclin D1 on paraffin embeddedsection of Human Mantle Cell Lymphomaãwith AM26674AF-N.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Cyclin D1 expression in HeLa cells. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26674AF-N to the cells.