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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP17815PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP17815PU-N, RRID:AB_10654514
- Product name
- anti Tyrosinase (TYR) (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 486-513 amino acids from the C-terminal region of Human Tyrosinase.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Tyrosinase antibody (C-term) (AP17815PU-N) in HepG2 cell line lysates (35ug/lane). Tyrosinase (arrow) was detected using the purified Pab.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Tyrosinase Antibody (C-term) Cat.-No AP17815PU-N in HepG2 cell line lysates (35ug/lane). Tyrosinase (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Tyrosinase Antibody (C-term) Cat.-No AP17815PU-N with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue using Tyrosinase Antibody (C-term) Cat.-No AP17815PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of Tyrosinase Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Fow Cytometric analysis of A375 cells using Tyrosinase Antibody (C-term) Cat.-No AP17815PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
