Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-17247 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal MDM2/HDM2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-κB and p53 signaling pathways.
Ullah K, Rosendahl AH, Izzi V, Bergmann U, Pihlajaniemi T, Mäki JM, Myllyharju J
Scientific reports 2017 Dec 8;7(1):17220
Scientific reports 2017 Dec 8;7(1):17220
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract G. 30 ug C2C12 whole cell ysate/extract 7.5 % SDS-PAGE MDM2 antibody dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Multiple bands were observed 60 ~ 97 kDa. It is possibly due to alternative splicing. Reference:PMID: 9840926
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MDM2/HDM2 Antibody [NBP2-17247] - IP samples: Jurkat whole cell extract A. 30 ug Jurkat cell whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of MDM2 protein by 4 ug MDM2 antibody 7.5 % SDS-PAGE The immunoprecipitated MDM2 protein was detected by MDM2 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MDM2 Antibody [NBP2-17247] - Immunohistochemical analysis of paraffin-embedded lung CA, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Paraffin-embedded human lung. MDM2 antibody diluted at 1:500.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: MDM2/HDM2 Antibody [NBP2-17247] - Cross-linked ChIP was performed with PC-3 chromatin extract and 5 ug of either control rabbit IgG or anti-MDM2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.