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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 600-401-423 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-423, RRID:AB_217947
- Product name
- Anti-MDM2 pS185 (RABBIT) Antibody - 600-401-423
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Affinity Purified Anti-MDM2 pS185 (Rabbit) is shown to detect a 102 kDa band (arrow) corresponding to phosphorylated mouse MDM2 present in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approxi-mately 20 µg of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
- Validation comment
- Western Blot
