Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-15145 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-CHK1 (Ser345) Monoclonal Antibody (S.48.4)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- S.48.4
- Vial size
- 100 µL
- Concentration
- 37 µg/mL
- Storage
- -20°C
Submitted references Replication Stress Induces Global Chromosome Breakage in the Fragile X Genome.
Evaluation of novel imidazotetrazine analogues designed to overcome temozolomide resistance and glioblastoma regrowth.
Chakraborty A, Jenjaroenpun P, Li J, El Hilali S, McCulley A, Haarer B, Hoffman EA, Belak A, Thorland A, Hehnly H, Schildkraut CL, Chen CL, Kuznetsov VA, Feng W
Cell reports 2020 Sep 22;32(12):108179
Cell reports 2020 Sep 22;32(12):108179
Evaluation of novel imidazotetrazine analogues designed to overcome temozolomide resistance and glioblastoma regrowth.
Ramirez YP, Mladek AC, Phillips RM, Gynther M, Rautio J, Ross AH, Wheelhouse RT, Sakaria JN
Molecular cancer therapeutics 2015 Jan;14(1):111-9
Molecular cancer therapeutics 2015 Jan;14(1):111-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), HeLa treated with Calyculin A (100nM, 1 hr) (Lane 2), HeLa (Lane 3), HeLa treated with Etoposide (5uM, 16 hrs) (Lane 4), U-2 OS (Lane 5), U-2 OS treated with Calyculin A (100nM, 1 hr) (Lane 6). The blot was probed with Anti-Phospho-CHK1 (Ser345) Monoclonal Antibody (S.48.4) (Product # MA5-15145, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). A 54kDa band corresponding to Phospho-CHK1 (Ser345) was observed in the treated cell lines.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-Chk1 pSer345 in C2C12 cells using a Phospho-Chk1 pSer345 monoclonal antibody (Product # MA5-15145) (green). Actin filaments are labeled with a fluorescent red phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Phospho-CHK1 (Ser345) was performed using 70% confluent log phase HeLa cells treated with Etoposide (5uM, 16 hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-CHK1 (Ser345) Monoclonal Antibody (S.48.4) (Product # MA5-15145) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing induction of expression upon treatment. Panel e represents control cells without treatment. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Phospho-Chk1 pSer345 in untreated (blue) and UV-treated (green) HeLa cells using a Phospho-Chk1 pSer345 monoclonal antibody (Product # MA5-15145).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL