16612-1-AP

antibody from Invitrogen Antibodies

Targeting: BCL2L13 BCL-RAMBO, MIL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16612-1-AP

Provider

Invitrogen Antibodies

Product name

BCL2L13 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MLLELTRRG QEPLSALLQF GVTYLEDYSA EYIIQQGGWG TVFSLESEEE EYPGITAEDS NDIYILPSDN SGQVSPPESP TVTTSWQSES LPVSLSASQS WHTESLPVSL GPESWQQIAM DPEEVKSLDS NGAGEKSENN SSNSDIVHVE KEEVPEGMEE AAVASVVLPA RELQEALPEA PAPLLPHITA TSLLGTREPD TEVITVEKSS PATSLFVELD EEEVKAATTE PTEVEEVVPA LEPTETLLSE KEINAREESL VEELSPASEK KPVPPSEGKS RLSPAGEMKP MPLSEGKSIL L (1-300 aa encoded by BC003032)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.24 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 16612-1-AP (BCL2L13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C6 cells were subjected to SDS PAGE followed by western blot with 16612-1-AP ( BCL2L13 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 16612-1-AP (BCL2L13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16612-1-AP (BCL2L13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using BCL2L13 antibody 16612-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas using 16612-1-AP (BCL2L13 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas using 16612-1-AP (BCL2L13 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using (Product # 16612-1-AP) (BCL2L13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-BCL2L13 (IP:16612-1-AP, 3ug; Detection:16612-1-AP 1:100) with mouse heart tissue lysate 8000ug.