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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- 12303-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDCD6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAAYSYRPG PGAGPGPAAG AALPDQSFLW NVFQRVDKDR SGVISDTELQ QALSNGTWTP FNPVTVRSII SMFDRENKAG VNFSEFTGVW KYITDWQNVF RTYDRDNSGM IDKNELKQAL SGFGYRLSDQ FHDILIRKFD RQGRGQIAFD DFIQGCIVLQ RLTDIFRRYD TDQDGWIQVS YEQYLSMVFS IV (1-191 aa encoded by BC012384)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.37 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12303-1-AP ( PDCD6 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12303-1-AP ( PDCD6 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PDCD6 (IP:12303-1-AP, 3ug; Detection:12303-1-AP 1:500) with HeLa cells lysate 3200ug.
