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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- NBP2-29454 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CEACAM/CD66 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody recognizes proteins of 80-200kDa, identified as different members of CEACAM family. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEACAM is not found in benign glands, stroma, or malignant prostatic cells
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
Submitted references Disulfide-HMGB1 Drives Ischemia-Reperfusion Injury in Human Liver Transplantation.
Sosa RA, Terry AQ, Kaldas FM, Jin YP, Rossetti M, Ito T, Li F, Ahn RS, Naini BV, Groysberg VM, Zheng Y, Aziz A, Nevarez-Mejia J, Zarrinpar A, Busuttil RW, Gjertson DW, Kupiec-Weglinski JW, Reed EF
Hepatology (Baltimore, Md.) 2020 May 19;
Hepatology (Baltimore, Md.) 2020 May 19;
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CEACAM/CD66 Antibody (COL-1) [NBP2-29454] - Analysis of CEA in human stomach lysate using CEA antibody at 2 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CEACAM/CD66 Antibody (COL-1) [NBP2-29454] - Formalin-fixed, paraffin-embedded normal human colon stained with CEA COL-1 antibody at 1:200 using peroxidase-conjugate and DAB chromogen.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CEACAM/CD66 Antibody (COL-1) [NBP2-29454] - Formalin-fixed, paraffin-embedded human colon cancer stained with CEA COL-1 antibody at 1:200 using peroxidase-conjugate and DAB chromogen.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CEACAM/CD66 Antibody (COL-1) [NBP2-29454] - Staining of colon cancer.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): CEACAM/CD66 Antibody (COL-1) [NBP2-29454] - An intracellular stain was performed on A549 cells with CEACAM/CD66 Antibody (COL-1) NBP2-33306PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE format of this antibody.
