Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 101-M181 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- CEACAM-1
- Antibody type
- Monoclonal
- Antigen
- recombinant human soluble CEACAM-1-Fc (produced in HEK293 cells)
- Description
- antibody purified from hybridoma supernatant
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- (#C5-1X)
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references Unaltered Fungal Burden and Lethality in Human CEACAM1-Transgenic Mice During Candida albicans Dissemination and Systemic Infection.
Binding of Candida albicans to Human CEACAM1 and CEACAM6 Modulates the Inflammatory Response of Intestinal Epithelial Cells.
CEACAM1 mediates B cell aggregation in central nervous system autoimmunity
Tumor and endothelial cell-derived microvesicles carry distinct CEACAMs and influence T-cell behavior.
Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons
Klaile E, Müller MM, Zubiría-Barrera C, Brehme S, Klassert TE, Stock M, Durotin A, Nguyen TD, Feer S, Singer BB, Zipfel PF, Rudolphi S, Jacobsen ID, Slevogt H
Frontiers in microbiology 2019;10:2703
Frontiers in microbiology 2019;10:2703
Binding of Candida albicans to Human CEACAM1 and CEACAM6 Modulates the Inflammatory Response of Intestinal Epithelial Cells.
Klaile E, Müller MM, Schäfer MR, Clauder AK, Feer S, Heyl KA, Stock M, Klassert TE, Zipfel PF, Singer BB, Slevogt H
mBio 2017 Mar 14;8(2)
mBio 2017 Mar 14;8(2)
CEACAM1 mediates B cell aggregation in central nervous system autoimmunity
Rovituso D, Scheffler L, Wunsch M, Kleinschnitz C, Dörck S, Ulzheimer J, Bayas A, Steinman L, Ergün S, Kuerten S
Scientific Reports 2016 September;6(1)
Scientific Reports 2016 September;6(1)
Tumor and endothelial cell-derived microvesicles carry distinct CEACAMs and influence T-cell behavior.
Muturi HT, Dreesen JD, Nilewski E, Jastrow H, Giebel B, Ergun S, Singer BB
PloS one 2013;8(9):e74654
PloS one 2013;8(9):e74654
Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons
Klaile E, Klassert T, Scheffrahn I, Müller M, Heinrich A, Heyl K, Dienemann H, Grünewald C, Bals R, Singer B, Slevogt H
Respiratory Research 2013 ;14(1):85
Respiratory Research 2013 ;14(1):85
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: Human CEACAM-1 lysate Detection utilizing 10 µg/ml.
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Sandwich ELISA: Solid phase was coated with 3 µg/ml anti CEA (Dako) binding human CEACAM-1 and CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody C5-1X (10 µg/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm. NOTE: In Sandwich ELISA C5-1X can also be used as catcher antibody.
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence with HeLa-CEACAM-1 cells. Cells were 4% PFA fixed (10min) and then incubated in 5% BSA/PBS for 1 h to block non-specific prtein-protein interactions. The cells were then incubated with 10µg C5-1X overnight at 4°C. The secondary antibody (green) was Alexa Flour® 488 goat-anti-mouse IgG (H+L).
- Sample type
- Hela-human CEACAM-1 cells
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- IHC staining of human jejunum tissue with C5-1X. CEACAM1 was detected in PFA-fixed paraffin-embedded sections of human jejunum tissue using mAb C5-1X followed by staining with anti-mouse HRP-DAB and counterstaining with hematoxylin. The labeling showed a clear staining of CEACAM1 by C5-1X.
- Sample type
- Human jejunum tissue
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Flow cytometry with stably transfected HeLa cells: 250.000 Hela-human CEACAM1 cells; 10 µg/ml of primary C5-1X. Binding was detected with a FITC-conjugated secondary antibody.
- Sample type
- Hela-human CEACAM-1 cells