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- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- 101-M182 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- CEACAM-1
- Antibody type
- Monoclonal
- Antigen
- recombinant human soluble CEACAM-1-Fc (produced in HEK293 cells)
- Description
- antibody purified from hybridoma supernatant
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- (#B3)
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references Binding of Candida albicans to Human CEACAM1 and CEACAM6 Modulates the Inflammatory Response of Intestinal Epithelial Cells.
CEACAM1 induces B-cell survival and is essential for protective antiviral antibody production.
Functional capacities of human IgM memory B cells in early inflammatory responses and secondary germinal center reactions.
Soluble CEACAM8 interacts with CEACAM1 inhibiting TLR2-triggered immune responses.
Tumor and endothelial cell-derived microvesicles carry distinct CEACAMs and influence T-cell behavior.
Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons
Klaile E, Müller MM, Schäfer MR, Clauder AK, Feer S, Heyl KA, Stock M, Klassert TE, Zipfel PF, Singer BB, Slevogt H
mBio 2017 Mar 14;8(2)
mBio 2017 Mar 14;8(2)
CEACAM1 induces B-cell survival and is essential for protective antiviral antibody production.
Khairnar V, Duhan V, Maney SK, Honke N, Shaabani N, Pandyra AA, Seifert M, Pozdeev V, Xu HC, Sharma P, Baldin F, Marquardsen F, Merches K, Lang E, Kirschning C, Westendorf AM, Häussinger D, Lang F, Dittmer U, Küppers R, Recher M, Hardt C, Scheffrahn I, Beauchemin N, Göthert JR, Singer BB, Lang PA, Lang KS
Nature communications 2015 Feb 18;6:6217
Nature communications 2015 Feb 18;6:6217
Functional capacities of human IgM memory B cells in early inflammatory responses and secondary germinal center reactions.
Seifert M, Przekopowitz M, Taudien S, Lollies A, Ronge V, Drees B, Lindemann M, Hillen U, Engler H, Singer BB, Küppers R
Proceedings of the National Academy of Sciences of the United States of America 2015 Feb 10;112(6):E546-55
Proceedings of the National Academy of Sciences of the United States of America 2015 Feb 10;112(6):E546-55
Soluble CEACAM8 interacts with CEACAM1 inhibiting TLR2-triggered immune responses.
Singer BB, Opp L, Heinrich A, Schreiber F, Binding-Liermann R, Berrocal-Almanza LC, Heyl KA, Müller MM, Weimann A, Zweigner J, Slevogt H
PloS one 2014;9(4):e94106
PloS one 2014;9(4):e94106
Tumor and endothelial cell-derived microvesicles carry distinct CEACAMs and influence T-cell behavior.
Muturi HT, Dreesen JD, Nilewski E, Jastrow H, Giebel B, Ergun S, Singer BB
PloS one 2013;8(9):e74654
PloS one 2013;8(9):e74654
Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons
Klaile E, Klassert T, Scheffrahn I, Müller M, Heinrich A, Heyl K, Dienemann H, Grünewald C, Bals R, Singer B, Slevogt H
Respiratory Research 2013 ;14(1):85
Respiratory Research 2013 ;14(1):85
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: Human CEACAM-1 lysate Detection utilizing 10 µg/ml.
- Sample type
- Cell lysate
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Sandwich ELISA: Solid phase was coated with 3 µg/ml anti CEA (Dako) binding human CEACAM-1 and CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody B3-17 (10 µg/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm. NOTE: In Sandwich ELISA B3-17 can also be used as catcher antibody.
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence with HeLa-CEACAM-1 cells. Cells were 4% PFA fixed (10min) and then incubated in 5% BSA/PBS for 1 h to block non-specific prtein-protein interactions. The cells were then incubated with 10µg B3-17 overnight at 4°C. The secondary antibody (green) was Alexa Flour® 488 goat-anti-mouse IgG (H+L).
- Sample type
- Hela-human CEACAM-1 cells
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- IHC staining of human jejunum tissue with mAb B3. CEACAM1 was detected in PFA-fixed paraffin-embedded sections of human jejunum tissue using mAb B3 followed by staining with anti-mouse HRP-DAB and counterstaining with hematoxylin. The labeling showed week staining of CEACAM1 by B3.
- Sample type
- Human jejunum tissue
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Flow cytometry with stably transfected HeLa cells: 250.000 Hela-human CEACAM1 cells; 10 µg/ml of primary B3-17. Binding was detected with a FITC-conjugated secondary antibody.
- Sample type
- Hela-human CEACAM-1 cells
