Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM26012PU-N - Provider product page
- Provider
- OriGene
- Product name
- CEACAM1 mouse monoclonal antibody, clone B3, Purified
- Antibody type
- Monoclonal
- Description
- CEACAM1 mouse monoclonal antibody, clone B3, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CEACAM1
- Isotype
- IgG
- Antibody clone number
- B3
- Vial size
- 100 µg
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis: Human CEACAM-1 lysate Detection utilysing 10 ug/ml
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sandwich ELISA: Solid phase was coated with 3 ug/ml anti CEA binding human CEACAM1- CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody mAb B3 (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence with HeLa-CEACAM-1 cells. Cells were 4% PFA fixed (10min) and then incubated in 5% BSA/PBS for 1 h to block non-specific prtein-protein interactions. The cells were then incubated with 10ug B3-17 overnight at 4°C. The secondary antibody (green) was Alexa Flour?488 goat-anti-Mouse IgG (H+L).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IHC staining of human jejunum tissue with Monoclonal Antibody B3. CEACAM1 was detected in PFA-fixed paraffin-embedded sections of human jejunum tissue using B3 followed by staining with anti-mouse HRP-DAB and counterstaining with hematoxylin. The labeling showed week staining of CEACAM1 by B3.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometry: 10 ug/ml of primary mAb B3; 250.000 Hela-human CEACAM1 cells
- Validation comment
- FC
