A700-090

antibody from Invitrogen Antibodies

Targeting: CEACAM1 BGP, BGP1, CD66a

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-090

Provider

Invitrogen Antibodies

Product name

CEACAM1 Recombinant Rabbit Monoclonal Antibody (BLR090G)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR090G

Vial size

100 µL

Concentration

1000 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CEACAM1 by western blot. Samples: Whole cell lysate (5 µg) from HEK293T, UACC-257, GaMG, Hep-G2, U2OS, A-549, SK-MEL-28, MCF-7, HT-29, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal antibody [BLR090G] (Product # A700-090 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR034F] (Product # A700-034 ).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CEACAM1 in FFPE Hep-G2 cells by ICC. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal antibody [BLR090G] (Product # A700-090; lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CEACAM1 in FFPE liver carcinoma by IHC. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal antibody [BLR090G] (Product # A700-090 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CEACAM1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal antibody (BLR090G) (A700-090 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CEACAM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from UACC-257 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CEACAM1 recombinant monoclonal antibody [BLR090G] (Product # A700-090 lot 1) used for IP at 6 µL per reaction. CEACAM1 was also immunoprecipitated by rabbit anti-CEACAM1/5 recombinant monoclonal antibody [BLR032F] (Product # A700-032). For blotting immunoprecipitated CEACAM1 (Product # A700-090) was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.