PA5-48065

antibody from Invitrogen Antibodies

Targeting: PLXNB3 PLEXB3, PLEXR, PLXN6

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-48065

Provider

Invitrogen Antibodies

Product name

PLXNB3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse Plexin B3 is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL Sheep Anti-human Plexin B3 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-48065) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. For additional reference, recombinant human Plexin B3 (1 ng) was included. A specific band for Plexin B3 was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PLXNB3 in rat embryonic hippocampal neurons. Samples were incubated in PLXNB3 polyclonal antibody (Product # PA5-48065) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. For additional reference, Recombinant Human Plexin B3 (1 ng) was included. A specific band for Plexin B3 was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of PLXNB3 in A172 human glioblastoma cell line. Samples were incubated in PLXNB3 polyclonal antibody (Product # PA5-48065) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody.