ADI-905-629-100

antibody from Enzo Life Sciences

Targeting: SPP1 BNSP, BSPI, ETA-1, OPN

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

ADI-905-629-100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ADI-905-629-100, RRID:AB_2039429

Product name

Osteopontin monoclonal antibody (53)

Antibody type

Monoclonal

Antigen

Recombinant full length protein

Description

Protein G affinity purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

53

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis: Protein samples (MDA-MB-435 cell lysates, conditioned medium from MDA-MB-435 cells containing secreted OPN or recombinant protein) were resolved by SDS-PAGE on a 8-16% gradient gel, transferred to nitrocellulose, and blocked for 2 hrs in Tris-buffered saline containing 0.05% Tween 20 (TBS-T) and 5% milk powder. For immunodetection, Blots were incubated with anti-OPN 53 (5µg/ml) overnight at 4¡C, followed by incubation with a HRP-conjugated goat anti-mouse secondary antibody (in TBS-T) for 1 hr at room temperature. Protein bands were visualized by enhanced chemiluminescence on Kodak X-OMAT film. M= Magic Mark.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded breast cancer tissue stained with 2µg/ml anti-OPN 53.