Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-2727 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2727, RRID:AB_10001077
- Product name
- Mouse Monoclonal Involucrin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Canine, Porcine, Simian
- Host
- Mouse
- Antigen sequence
The epitope maps between codon 421-
568 of human involucrin.- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Changes in ceramides and glucosylceramides in mouse skin and human epidermal equivalents by rice-derived glucosylceramide.
Shimoda H, Terazawa S, Hitoe S, Tanaka J, Nakamura S, Matsuda H, Yoshikawa M
Journal of medicinal food 2012 Dec;15(12):1064-72
Journal of medicinal food 2012 Dec;15(12):1064-72
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Involucrin Antibody (SY5) [NB100-2727] - Involucrin from primary human epidermal keratinocyte lysates grown in low calcium (0.07 mM) for 5 days or differentiated with high calcium (1.5 mM) for 1-5 days. Block in 5% nonfat dry milk in TBST incubated at RT for 1 hr, antibody 1:500 in TBST, overnight at 4C. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Involucrin Antibody (SY5) [NB100-2727] - Staining of human Tonsil with Involucrin Monoclonal Antibody (SY5).
